Part:BBa_K531005:Design

P_xyl promoter+optimized esp+rsaA secretion tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 391
  Illegal XhoI site found at 140
  Illegal XhoI site found at 961
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1214
  Illegal AgeI site found at 1337
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

no stop codon in esp gene and no start codon in rsaA gene

Source

From BBa_K531001 BBa_K531002 BBa_K531003